Product description this document summarizes the procedures for installing and using the veriti thermal cycler. Applied biosystems veriti thermal cycler user guide pn 4375799. The applied biosystems veriti 96well thermal cycler delivers the proven reliability of applied biosystems instruments. This personalsized 96well thermal cycler is ideal for both basic pcr and cyclesequencing applications using 0. C pcr results showing six primer sets run in a single pcr ampli. The simpliamp thermal cycler is a small, easytouse, and accurate thermal cycler that suits any labs needs for everyday pcr. Miniamp, simpliamp, proflex, and veriti thermal cyclers have thermal simulation modes that make the transition from other thermal cyclers simple, accurate, and efficient. When samples are ready, tray of tubes are put directly on block of veriti 96well thermal cycler eliminates hassle of transferring tubes and provides thermal contact necessary for thermal cycling see user s manual or package insert for limited label license and trademark information. Veriti pcr system installation and operation fisher scientific.
Miniamp and miniamp plus thermal cycler user guide 11. Veriti thermal cycler this abi thermal cycler performs standard pcr, including temperature gradients, and it is protected from brief power outages by an apc backup power supply. Additionally, the large navigation buttons put programming of the veriti thermal cycler at your finger tips. Pcr thermal cyclers support troubleshooting thermo. Normally, all other thermocyclers in the genomics facility are reserved solely for use by the facility or members of the batzer lab. With the veriti thermal cycler you get two thermal cyclers in one, a standard thermal cycler with its better than gradient feature, and a fast enabled thermal cycler. Applied biosystems veriti thermal cycler user guide, 2010. With its robust design, the t100 system is a reliable personal thermal cycler that delivers exceptional performance for years launch the system tour. Procedures should be performed when instrument has been relocated or modifications have been made to the instruments configuration. Veriti 96well fast thermal cycler 4375305 a27924 veriti 96well thermal cycler 4375786 a26659 veriti 384well thermal cycler 4388444 a27927. Atflsfb29 quality control of instruments and equipment. The user manual provides information about performing runs and diagnostic tests as well as maintenance and troubleshooting. Functions that are not the same for each model are indicated by a comment.
Fast ramping thermal cyclers enable an 8hour massarray. Accurate temperature measurement verification kits alpha. Please select your current applied biosystems thermal cycler. This user guide provides information on how to maximize the capabilities of your new veritithermal cycler. Audiencethis guide is intended for novice and experienced veriti thermal cycler users who use the instrument to perform pcr. Use care when working around the heated cover and sample blocks to avoid being burned. The applied biosystems veriti user guideprovides information about installing, using and maintaining the applied biosystems veriti thermal cycler. Wear appropriate protective eyewear, clothing, and gloves. C touchtm thermal cycler manual iii safety and regulatory compliance the c touch thermal cycler heats and cools very quickly during operation. Multiprobe tvk user guide for veriti thermal cycler. Usb b port connects the c touch thermal cycler to a computer usb a ports connects up to three s thermal cyclers to the c touch thermal cycler. Product description this document summarizes procedures for installing and using the veriti pro thermal cycler with 6zone 96well 0.
Applied biosystems microamp 96well trayretainer set for. For more information on the veriti systems, see the. The multiprobe tvks must be used only by operators trained in laboratory. Detailed specifications for the veritithermal cycler are found in appendix a, instrument specifications. Multiprobe tvk user guide for veriti dx thermal cycler, 16 veriti dx thermal cycler before you run 96well instrument tests physical injury hazard. Accurate temperature measurement verification kits. Learn about the applied biosystems veriti thermal cycler. Thermal cyclers are essential laboratory equipment for researchers who run polymerase chain reaction pcr for sequencing, cloning, genotyping, mutagenesis, and many other applications. The miniamp thermal cycler and the miniamp plus thermal cycler are endpoint thermal cyclers, specifically designed for the amplification of nucleic acids using the polymerase chain reaction pcr process. The applied biosystems veriti user guideprovides information about installing, using and maintaining the applied biosystems veritithermal cycler. Veriti dx thermal cycler user guide thermo fisher scientific. Audience this guide is intended for novice and experienced veriti thermal cycler users who. Microamp for use with equipment 2720 thermal cycler, 3500xl dx genetic analyzer, 7000 system, 7300 system, 7500 fast system, 7500 system, 7900ht fast system, 7900ht system, ab 2720 thermal cycler, geneamp 9700, quantstudio, simpliamp thermal cycler, steponeplus, veriti dx.
Read the safety data sheets sdss and follow the handling instructions. The applied biosystems 2720 thermal cycler user manual describes how to use the applied biosystems 2720 thermal cycler. Control at your fingertips the powerful, yet simple to operate user interface on the veriti system is driven by the 6. The software product contains trade secrets and in order to protect them he may not decompile, reverse engineer, disassemble, or otherwise reduce the software product to a human perceivable form or create derivative. Additionally, the large navigation buttons put programming of the veriti dx thermal cycler at your fingertips. Description the applied biosystems veriti 96well thermal cycler delivers the proven reliability of applied biosystems instruments. Applied biosystems microamp reaction tube without cap, 0. Veriti 96well thermal cycler from applied biosystems. For detailed instructions, see the veritithermal cycler user guide pub. Industrial carrying case, mineral oil and cotton swabs. Applied biosystems microamp 96well support base life. This document summarizes the procedures for installing and using the veriti thermal cycler.
Veriti pcr system installation and operation quick. In laboratories regulated typically by gxp and iso, in compliance with government and international standards requires documented verification that your systems are installed and functioning according to their operational specifications. Use of support services is governed by the cyclertest bv policies and programs described in the user manual, in online documentation, andor. The veritipro thermal cycler is an endpoint thermal cyclers, specifically designed for the amplification of nucleic acids using the polymerase chain reaction pcr process. We strongly recommend that you follow the safety specifications listed in this section and throughout this manual. It is crafted with the reliable engineering and performance youve come to expect from applied biosystem instruments. This manual contains a protocol for use of the powerplex fusion 6c system with the geneamp pcr system 9700, veriti 96well thermal cycler and proflex pcr system in addition to protocols to separate amplified products and detect separated material on the. This feature is better than the standard gradient control method used by most. C touchtmthermal cycler manual 2 the back panel of the c touch thermal cycler includes data ports figure 2. The user interface includes a touchscreen with a graphical display that shows the time, status, and temperature for each run. Applied biosystems simpliamp thermal cycler reuzeit. Applied biosystems pcr thermal cyclers and plastics. Applied biosystems microamp 96well support base 10 bases.
Powerplexr fusion 6c system for use on the applied. Page 1 simpliamp thermal cycler user guide installation, use, and maintenance for use with. Cyclers can, depending on brand, have a high variation in block wells, block angles and dimensions. Veriti dx thermal cycler thermo fisher scientific us. Veriti thermal cycler user guide vii preface how to use this guide purpose of this guide the applied biosystems veriti user guide provides information about installing, using and maintaining the applied biosystems veriti thermal cycler. Available in three formats to suit your thermal cycling needs. Veriti thermal cycler, iq userperformed test procedures. Page 2 the information in this guide is subject to change without notice. Veritipro product description thermal cycler installation and. A library of modes that mimic the ramp rates of other instruments is available on each instrument. Our first endpoint thermal cycler for in vitro diagnostic use.
Product information the simpliamp thermal cycler is an endpoint thermal cycler, specifically designed for the amplification of nucleic acids using the polymerase chain reaction pcr process. Functions which are not the same for each model are indicated. This user guide provides information on how to maximize the capabilities of this system. The abi veriti 96 thermal cycler is easy to use and powerful with an intuitive color touch screen controller. Airclean systems ductless pcr workstation operators manual, 2006. Our first endpoint thermal cycler for in vitro diagnostic use the applied biosystems veriti dx thermal cycler is classified as a us fda class i medical device. Applied biosystem 2720 thermal cycler 96well block.
Appendix a for decontamination and shipping instructions. Applied biosystems veriti thermal cycler, 60well veriti. Applied biosystems veriti thermal cyclers user manualjapanese. The applied biosystems veriti dx thermal cycler is classified as a us fda class i medical device. See set up the miniamp or miniamp plus thermal cycler on page 7. The massarray workflow can be accomplished in 8 hours using the veriti thermal cycler. The veriti thermal cycler reduces the workflow time by nearly 2 hours. Thermal cyclers for pcr life science research biorad. Miniamp and miniamp plus thermal cycler user guide pub. With the added control of veriflex technology, you have six independent temperature blocks that provide precise control over your pcr optimization. This user guide provides instructions for all models of the. Applied biosystems veriti 96well thermal cycler 1 x 96well.
Sep 15, 2008 the veriti thermal cycler from applied biosystems is specifically designed to meet the needs of labs performing standard pcr along with pcr optimization in the 96well format. Set up the system for instructions on connecting the instrument to the network. The abi veriti 96 well thermal cycler comes with veriflex blocks that allow you to run six independent temperatures, giving the user precision control, and the ability to run multiple experiments. Invitrogen and applied biosystems pcr reagents catalog number a24811 publication number man0009889 revision c. We performed statistical comparisons of the two thermal cyclers to ensure consistent results with the geneamp thermal cyclers and with the veriti 96well thermal cycler with 0. For detailed instructions on using the thermal cycler, see the veritiprothermal cycler user guidepub. The powerful yet simpletooperate user interface on the veriti dx 96well thermal cycler is driven by the 6. Microamp for use with equipment 2720 thermal cycler, 3500xl dx genetic analyzer, 7000 system, 7300 system, 7500 fast system, 7500 system, 7900ht fast system, 7900ht system, ab 2720 thermal cycler, geneamp 9700, quantstudio, simpliamp thermal cycler, steponeplus, veriti. The large screen allows for easy viewing of your temperature profiles. A applied biosystems veriti 96well thermal cycler software shows annealing temperatures set for six different primers. So whether you have a current need to run fast pcr and shorten your pcr cycling times, or a possible future application need for fast pcr, the veriti thermal cycler provides you with the flexibility to go fast when you want. See user s manual or package insert for limited label license and trademark information.
Applied biosystems veriti 96well thermal cycler fisher. Abi veriti 96 well pcr machine the lab world group. The instrument is manufactured to iso 485 and gmp requirements. This compact thermal cycler features an intuitive touchscreen user interface to make running pcr easy. The veriti thermal cycler is controlled using a touchscreen display with easytouse interface. For more information, see the veriti thermal cycler. With this enhanced functionality, the veriti 96well thermal cycler with veriflex blocks is the ideal thermal cycler for any lab. Thermal gradient technology allows you to quickly optimize your reaction in a single run. In the home screen for the touchscreen, you can set up a run by creating a new method for a run. Applied biosystems simpliamp thermal cy a24812 andbio. Veriti pcr system installation and operation quick reference. Different kits are available for different thermal cyclers.
In summary, the veriti thermal cycler is highly recommended as a replacement for the geneamp 9700 thermal cycler for those who wish to reduce the overall cycling time and process. This iq installation qualification userperformed test procedures package provides you with test procedures to document that your veriti thermal cycler is installed to manufacturers specifications. Applied biosystems veriti thermal cycler user guide veriti thermal applied biosystems touching. Audiencethis guide is intended for novice and experienced veritithermal cycler users who use the instrument to perform pcr. Extension 50c 20 min hot start 95c 5 min pcr cycle 95c 30 s 54c 1 min. Veriti 96well thermal cycler thermo fisher scientific. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large lcd color touch screen. The multiprobe tvks verify the temperature accuracy and nonuniformity of applied biosystems thermal cyclers. Common features of biorads thermal cyclers include peltier effect technology for precise temperature control, and thermal gradient to easily optimize pcr.
Information can also be found in the veritithermal cycler help accessed by pressing in the touchscreen. For detailed specifications of the veritipro thermal cycler, see appendix c, instrument specification and layout. Up to twelve veriti thermal cyclers can be connected in a satellitelike format through a network switch. Applied biosystems simpliamp thermal cycler user manual pdf. Text conventionsthis guide uses the following conventions. This user guide provides instructions for all four models of the veriti thermal cycler. It is also compatible with the applied biosystems thermal cycler fleet control software. Figure 11 the applied biosystems veritithermal cycler. Pcr thermal cyclers support troubleshooting thermo fisher. Multiprobe temperature verification kit for use with veriti thermal. Catalog numbers 4375305, 4375786, 4384638, 4388444, 44751, 44964, and zgexscverti3y. Thermal cycler veriti thermal cycler 2720 thermal cycler automated thermal cycler max.
The veriti is fastenabled which means that the system has the flexibility to run fast chemistries. Applied biosystems veriti thermal cycler, 60well veriti 60. Appendix c, instrument specification and layout and layout. Veritipro product description thermal cycler installation. The powerful, yet simple to operate user interface on the veriti system is driven by the 6. Abi veriti 384 well thermal cycler is available from the lab world group. The applied biosystems veriti 96well thermal cycler delivers the proven. The user shall not rent, distribute or sublicense the original or the backup copy of the software product and the manuals.
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